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    For example, I have a bispecific Ab , immobilize it on the chip, then inject two antigens. Especially about how can I correctly choose the concentration? Hello, see nice references attached. Do you have any experience on it? Hello, provide more info what really you want. Darbinyan I want to test the binding of a bispecific antibody with two antigens simutanously, i. I Can sent you additional Material regarding ABA via mail. Join ResearchGate to ask questions, get input, and advance your work. I want to test the binding of a bispecific antibody with two antigens simutanously, i. How to experimentally determine the biosafety level of an environmental bacterial isolate? I am trying to adapt an existing method used on the Biacore3000 before to this new instrument. Biacore and this instrument and got the same results? How do you recommend adjusting the flow rate? Is negative zeta potential suitable for liposomes or positive zeta potential? Some articles have suggested that a negative zeta potential is appropriate for the liposome, and some articles have suggested that a positive zeta potential is a more appropriate factor for liposome delivery of the drug. What is end capping in short peptides such as pentapeptides or hexapeptides? I have come across the following word end capping in pentapeptides. Does it mean fixing the Ramachandran angles of end amino acids? Desalting Columns by GE to do a buffer exchange of my protein, and I have encountered some difficulties. Can someone please, step by step, shed some light on this topic? We are not sure what the problem might be. At first, we thought something was wrong with the Elution Buffer, but we prepared new batches, tried again and got the same result. Same for trying a different HiTrap Column. We are a little bit clueless and hope someone might have an idea. Can two EC50 values with different hill slopes be compared? The model I use is purely qualitative but it fits the data well. Does the EC50 value depend on the Hill slope? What is diffrence between coupling buffer and immobilization buffer in SPR experiment? SPR experiment and i am referring some journal and websites they stated regarding coupling buffer and immobilization buffer so ihave confusion about these buffer. Surface plasmon resonance measurements to quantify affinity values were made using a Biacore X100 surface plasmon resonance instrument. BSA was similarly immobilized to the control channel. Kis antitoxin to the CcdB toxin analyzed by SPR. CcdB interactions obtained in a Biacore 3000 flowing different concentrations of the His6Kis antitoxin on the toxin immobilized on the chip. Basic operations and analysis were as indicated in Material and Methods. Join ResearchGate to find the people and research you need to help your work. Please send this request first and then create another cart. Please note, up to 20 product numbers can be added to a single quote request. We were not able to load your agreed MSA pricing. You can still process your order and the final price will be shown on your invoice, or click here to try again. Please update your quantity to meet the minimum order quantity for each item. If you do not update your quantity, a customer service representative will get in touch with you before processing your order. If the problem persists contact the administrator. Something went wrong on the server. Choose another country or region to see specific content for your location and shop online. filexlib.
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